|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13736||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)840
Entrez GeneSIRT3 (a.k.a. SIR2L3)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Catalytic domain of SIRT3. Residues 357-1196. Molecular Weight: 31102 Da.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SIRT3L-12 was a gift from John Denu (Addgene plasmid # 13736 ; http://n2t.net/addgene:13736 ; RRID:Addgene_13736)
For your References section:Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548