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SIRT5-pET15b
(Plasmid #81030)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 81030 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sirt5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    825
  • GenBank ID
    23408
  • Entrez Gene
    SIRT5 (a.k.a. SIR2L5)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Drs. Marcia Haigis and Natalie German, Harvard Medical School

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert doesn't have mitochondrial targeting sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIRT5-pET15b was a gift from David Lombard (Addgene plasmid # 81030 ; http://n2t.net/addgene:81030 ; RRID:Addgene_81030)
  • For your References section:

    Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein. Kumar S, Lombard DB. Methods Mol Biol. 2016;1436:241-57. doi: 10.1007/978-1-4939-3667-0_16. 10.1007/978-1-4939-3667-0_16 PubMed 27246219