Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13737||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)940
Entrez GeneSIRT4 (a.k.a. SIR2L4)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pQE forward (5'-CCCGAAAAGTGCCACCTG-3') (Common Sequencing Primers)
Terms and Licenses
Full length SIRT4. Molecular Weight: 35188 Da.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SIRT4.23 was a gift from John Denu (Addgene plasmid # 13737 ; http://n2t.net/addgene:13737 ; RRID:Addgene_13737)
For your References section:Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548