pcDNA3.1his p66shc S36A
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10973||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 His
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSHC1 (a.k.a. SHC, SHCA)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this plasmid contains additional mutations (D2N and S38P) in the p66/SHC sequence when compared to GenBank entries NP_892113.4 and NP_001123512.1. This is the original plasmid as used in the associated publication listed below.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1his p66shc S36A was a gift from Toren Finkel (Addgene plasmid # 10973 ; http://n2t.net/addgene:10973 ; RRID:Addgene_10973)
For your References section:Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Nemoto S, Finkel T. Science. 2002 Mar 29. 295(5564):2450-2. 10.1126/science.1069004 PubMed 11884717