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Depositing Lab
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Depositing OrganizationWhitehead Institute for Biomedical Research
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 10908-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 10908-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCI-neo
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSnoN
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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Entrez GeneSKIL (a.k.a. SNO, SnoA, SnoI, SnoN)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Shunsuke Ishii
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SnoN was excised from a psp65 vector from S. Ishii. SnoN was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of SnoN directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.
DNA (Catalog # 10908-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 10908-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Neo HA-hSnoN was a gift from Bob Weinberg (Addgene plasmid # 10908 ; http://n2t.net/addgene:10908 ; RRID:Addgene_10908) -
For your References section:
SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling. Sun Y, Liu X, Ng-Eaton E, Lodish HF, Weinberg RA. Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7. 10.1073/pnas.96.22.12442 PubMed 10535941