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pCI-Neo HA-hSki
(Plasmid #10910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10910 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 10910-D50 50 μg of DNA in Tris buffer $495
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DNA 10910-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ski
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    NM_003036.3
  • Entrez Gene
    SKI (a.k.a. SGS, SKV)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shunsuke Ishii

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ski was excised from a psp65 vector from S. Ishii. Ski was cloned into pCI-Neo-HA at the SmaI site. But the HA tag was later engineered to adjoin the ATG of Ski directly without intervening vector sequence. So the insert can be cut out with XhoI and HpaI.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo HA-hSki was a gift from Bob Weinberg (Addgene plasmid # 10910 ; http://n2t.net/addgene:10910 ; RRID:Addgene_10910)

  • For your References section:

    SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling. Sun Y, Liu X, Ng-Eaton E, Lodish HF, Weinberg RA. Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12442-7. 10.1073/pnas.96.22.12442 PubMed 10535941
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