MFSD12 mediates the import of cysteine into melanosomes and lysosomes.
Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, Lewis CA, Sabatini DM
Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 10.1038/s41586-020-2937-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149504 | prk5-MFSD12-TEV-3xFLAG-3xHA | Transient expression of human MFSD12 with recoded nucleotide sequence. |
| 149506 | prk5-TMEM192-3xHA PM mutant | Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA. |
| 149507 | prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant | Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA. |
| 149508 | pCHA1.1-MFSD12-V5 | Stable expression of human MFSD12 with recoded nucleotide sequence. |
| 149509 | pCHA1.1-Rap2a-V5 | Stable expression of human Rap2a. |
| 160368 | MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc) | Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO |
| 160369 | MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA) | Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes. |
| 163126 | LentiCRISPRv2-Opti | LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell. |