Purpose(Empty Backbone) Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138086||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8684
Vector typeMammalian Expression, Lentiviral
- Promoter minimal CMV promoter with heptamerized tet‐operator
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer TCCACGCTGTTTTGACCTC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The plasmid can be used in conjunction with pTet-CTRL (Addgene #138085) that expresses the Tet-transactivator proteins for controlled expression of the ORF for the gene of interest cloned into this plasmid. Following Lentiviral transduction into mammalian cells expression can be induced with Tetracycline or Doxycyline.
To clone the gene of interest into this plasmid, digest with BamHI and then perform Gibson assembly, adding your gene of interest including any tags.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-Tet was a gift from Eva Frickel (Addgene plasmid # 138086 ; http://n2t.net/addgene:138086 ; RRID:Addgene_138086)
For your References section:Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM. EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. Epub 2019 Jun 3. 10.15252/embj.2018100926 PubMed 31268602