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pRK5_msfGFP-HMGB1-Shuffled 8

Plasmid

#237656

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 8

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 8 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Mut-C16toA

Plasmid

#237627

Purpose

For overexpression of msfGFP-HMGB1-Mut-C16toA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Mut-C16toA (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

C16A

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Mut-MtoED

Plasmid

#237628

Purpose

For overexpression of msfGFP-HMGB1-Mut-MtoED

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Mut-MtoED (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

M to ET

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Mut-0F

Plasmid

#237622

Purpose

For overexpression of msfGFP-HMGB1-Mut-0F

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Mut-0F (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

Remove all F

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Mut-F13A

Plasmid

#237624

Purpose

For overexpression of msfGFP-HMGB1-Mut-F13A

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Mut-F13A (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

F13A

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 6

Plasmid

#237654

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 6 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 1

Plasmid

#237649

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 1 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 2

Plasmid

#237650

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 2 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 10

Plasmid

#237658

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 10

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 10 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9ABEmax8.17d

Plasmid

#201955

Purpose

Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-G671R-N865K

Plasmid

#203699

Purpose

expresses ATP10B with the pathogenic mutations G671R and N865K

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

G671R, N865K

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

Plasmid

#194016

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.2)
eGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

cmb and u6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-79

Plasmid

#197397

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-79

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-79 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-59

Plasmid

#197396

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-59 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-His-zBasic-RBD-Avi

Plasmid

#195000

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

Depositor

Marko Hyvönen

Article

Fischer et al Nat Biotechnol. 2025 Jun;43(6):960-970.

Insert

RBD (S SARS-CoV-2)

Tags

Avi-tag and His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192243

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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