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pET28_hTLN2-head

Plasmid

#164836

Purpose

Expression of human talin2 head

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI3

Plasmid

#42527

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Mutation

gene sequence is codon optimized for expression i…

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC-CTHF

Plasmid

#26105

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

TEV - His6 - Flag

Expression

Bacterial

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pMycoFos

Plasmid

#84577

Purpose

Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

Depositor

Nicholas Coleman

Article

Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

Type

Empty backbone

Expression

Bacterial

Available Since

April 7, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGP4G-TPLRD1-ccdB

Plasmid

#83877

Purpose

Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGP4GY-cccdB

Plasmid

#83878

Purpose

Destination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Rap2b

Plasmid

#118321

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-T24N-mRFP1-polyA

Plasmid

#104561

Purpose

Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

T24N

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-myrGFP

Plasmid

#115502

Purpose

expression of myristoylated, plasma membrane targeted GFP

Depositor

Thomas Biederer

Article

Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

Insert

myristoylated GFP

Expression

Mammalian

Promoter

chicken beta-actin

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-) FLIPQTV3.0 2m

Plasmid

#63731

Purpose

FLIPQTV3.0 glutamine sensor with R75MW220A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 2mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Article

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV.IRES.hCD4

Plasmid

#35712

Depositor

Patricia Ernst

Article

Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

IRES
hCD4

Use

Retroviral

Expression

Mammalian

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPI356

Plasmid

#207449

Purpose

Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Inserts

PkgE
PTEN

Use

Unspecified

Tags

GFP and mCherry

Mutation

PH domain only

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 8m

Plasmid

#63732

Purpose

FLIPQTV3.0 glutamine sensor with R75MY86A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 8mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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