167,910 results

1. pET11d-RAN

   Plasmid

   #108919

   Purpose

   Expresses human RAN

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   RAN (RAN Human)
   

   Expression

   Bacterial

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-V5-Zeo

   Plasmid

   #207741

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. PB_TRE_AP4-PDGFR_EF1a_eBFP2

   Plasmid

   #186306

   Purpose

   Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP4-PDGFR
   eBFP2
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-3xGFP-tDeg

   Plasmid

   #191371

   Purpose

   Overexpression of 3xGFP-tDeg in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   3xGFP-tDeg
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-Halo-tDeg

   Plasmid

   #191370

   Purpose

   Overexpression of Halo-tDeg in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   Halo-tDeg
   

   Tags

   Halo

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-Akt-FoxO3a-KTR-EGFP

   Plasmid

   #125131

   Purpose

   Fluorescent reporter for Akt activity

   Depositor

   Kazuhiro Aoki

   Article

   Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

   Insert

   FOXO2 (FOXO3 Human)
   

   Tags

   Enhanced green fluorescent protein (EGFP)

   Expression

   Mammalian

   Mutation

   FoxO3A 1-402 amino acid

   Promoter

   CAG promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP3545-dCas9Str

   Plasmid

   #153516

   Purpose

   Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   dCas9Str
   

   Expression

   Bacterial

   Promoter

   nisA

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Rab5a-7

   Plasmid

   #55126

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Rubicon

   Plasmid

   #28022

   Depositor

   Qing Zhong

   Article

   Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

   Insert

   Rubicon (RUBCN Human)
   

   Tags

   3xFlag and EGFP

   Expression

   Mammalian

   Available Since

   March 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-H2B-miRFP670

    Plasmid

    #212668

    Purpose

    Express tagged H2B

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    miRFP670

    Promoter

    EF-1a

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLC-Flag-CRBN-P2A-Hygro

    Plasmid

    #124303

    Purpose

    Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CRBN (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.R_TRE_minCMV

    Plasmid

    #211518

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minCMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A

    Pooled Library

    #92379

    Purpose

    CRISPRa

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Has Service

    Lentiviral Prep

    Use

    Lentiviral

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

    Plasmid

    #203221

    Purpose

    Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCasMINI-V4-NFZ-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR221-Renilla

    Plasmid

    #79496

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Renilla Luciferase
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0120

    Plasmid

    #142539

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELMSAN1 (MIDEAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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