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pLminP_Luc2P_RE56

Plasmid

#247034

Purpose

Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NR3C1 (GR)

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP1 POD-nAb-WPRE

Plasmid

#244973

Purpose

Expresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

GFP1 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SGSM3[1-723]

Plasmid

#246258

Purpose

Rab5 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

Sgsm3[1-723] (Sgsm3 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-MLH1dn (mouse) for IVT

Plasmid

#246485

Purpose

Template for in vitro transcription of mouse MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (Mlh1 Mouse)

Use

Template for in vitro transcription

Mutation

deletion of residues 758-760

Promoter

T7

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9-KOX1

Plasmid

#240528

Purpose

Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9-KOX1

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-GST-Tev-Af1521-myc

Plasmid

#196239

Purpose

N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

N-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag

Tags

GST tag and myc tag

Expression

Bacterial

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-HOXA9-KS

Plasmid

#237673

Purpose

For overexpression of mEGFP-NUP98-HOXA9-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-HOXA9-KS (NUP98, HOXA9 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LbNOX-Flag

Plasmid

#234984

Purpose

Expresses LbNOX in human cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR4-V5

Plasmid

#236017

Purpose

for PiggyBac mediated integration and stable expression of hFGFR4 protein

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR4 (FGFR4 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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mNG-PH-CYTH3-2G

Plasmid

#233261

Purpose

Expression of mNeonGreen-tagged PIP3 reporter

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PIP3 reporter

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2480

Plasmid

#142102

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MXD4 (MXD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE

Plasmid

#226380

Purpose

Expresses tau propagation ORF with no V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T-2A

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.Tau2N4R

Plasmid

#226399

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-GEM

Plasmid

#187648

Purpose

Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

PfV

Use

AAV

Tags

HaloTag and SNAP-tag

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-tTA

Plasmid

#178516

Purpose

AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

tTA

Use

AAV

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

Plasmid

#200465

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(46) (SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EC67904

Plasmid

#209470

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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