165,586 results

1. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163476

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Beta Globin minimal promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Use

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b_Bst-LF

   Plasmid

   #153313

   Purpose

   Bacterial expression of the Bst Large Fragment

   Depositor

   Philippa Marrack, Drew Endy

   Insert

   Bst Large Fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mEGFP-H4-23

   Plasmid

   #56463

   Purpose

   Localization: Nucleus/Histones, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   H4 (HIST1H4J Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. rEstus

   Plasmid

   #209767

   Purpose

   To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

   Depositor

   Stefan Heinemann

   Article

   Rühl et al bioRxiv 2023.

   Insert

   rEstus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF806-reci

   Plasmid

   #186711

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Syn-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125693

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn1-eYFP (AAV9)

   Viral Prep

   #117382-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   Syn

   Tags

   eYFP

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pLX311_CdTag-HA_EmptyVector

   Plasmid

   #221468

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

    Plasmid

    #132543

    Purpose

    Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv d0

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV_DENV2_PrME

    Plasmid

    #207264

    Purpose

    Mammalian expression of Dengue virus 2 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV2_PrME (POLY Dengue virus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-6xGLY-Myc-hphMX4

    Plasmid

    #20770

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. EYFP Peroxisome visual barcode

    Plasmid

    #158677

    Purpose

    tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AARS_C2_pNIC-Bio3

    Plasmid

    #153047

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-6xGLY-FLAG-hphMX4

    Plasmid

    #20752

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLRT

    Plasmid

    #78234

    Purpose

    Expresses Murine Leukemia Reverse Transcriptase (MLRT)

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Insert

    Murine leukemia reverse transcriptase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mouse genome-wide lentiviral CRISPR gRNA library version 2

    Pooled Library

    #67988

    Purpose

    Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Species

    Homo sapiens, Mus musculus, and Synthetic

    Use

    CRISPR and Lentiviral

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only