167,825 results

1. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAXY0013

   Plasmid

   #205582

   Purpose

   NpuDnaE(C)_HYG(53-341)_RUBY

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

   Insert

   NpuDnaE(C)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Kif5c-Myc

   Plasmid

   #186616

   Purpose

   Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Heavy Chain isoform 5c (KIF5C Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3s-Cas9-HN

   Plasmid

   #104171

   Purpose

   Cas9-WT mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   Cas9-WT
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-BFP

   Plasmid

   #138272

   Purpose

   Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BFP H66 PAM
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   H66, PAM motif

   Promoter

   Ef1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138133

   Purpose

   Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4.TO-ORF37-2xCSTREP

   Plasmid

   #136198

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF37
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mARGAna (cassette 1)

   Plasmid

   #191341

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Expression

   Mammalian

   Promoter

   TRE3GV

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Linear-GFP

    Plasmid

    #226258

    Purpose

    Production of linear RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-hygro-eGFP-HEC1

    Plasmid

    #114027

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-EGFR-L858R

    Plasmid

    #116276

    Purpose

    Lentiviral expression of EGFR L858R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Mutation

    L858R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSA1_His

    Plasmid

    #202763

    Purpose

    human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA1
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-BclXL-pEGFP-C1

    Plasmid

    #177409

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-XL
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF11-wmiRFP680-T2A-HO1

    Plasmid

    #197244

    Purpose

    Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP680-T2A-HO1
    

    Expression

    Worm

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM mouse dbx-1 [TJ#11]

    Plasmid

    #16269

    Depositor

    Thomas Jessell

    Article

    Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

    Insert

    dbx1 3'UTR (Dbx1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only