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pl-Synapsin-YFP-NaVII-III
(Plasmid #91246)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91246 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
  • Backbone manufacturer
    Addgene plasmid 20945
  • Backbone size w/o insert (bp) 10093
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nav1.2
  • Alt name
    sodium channel subunit Nav1.2
  • Alt name
    sodium channel, voltage-gated
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    684
  • Mutation
    The insert codes only for the intracellular loop between transmembrane domains II and III
  • Entrez Gene
    Scn2a (a.k.a. NachII, Nav1.2, RII/RIIA, RNSCPIIR, SCN, Scn2a1, Scn2a2, ScpII)
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Resource Information

Depositor Comments

Please see the linked article - Dumitrescu et al. (2016) - for full details of the cloning procedure used to generate this consruct

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pl-Synapsin-YFP-NaVII-III was a gift from Matthew Grubb (Addgene plasmid # 91246)
  • For your References section:

    Evaluating Tools for Live Imaging of Structural Plasticity at the Axon Initial Segment. Dumitrescu AS, Evans MD, Grubb MS. Front Cell Neurosci. 2016 Nov 23;10:268. eCollection 2016. 10.3389/fncel.2016.00268 PubMed 27932952