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(Plasmid #162279)


Item Catalog # Description Quantity Price (USD)
Plasmid 162279 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6151
  • Total vector size (bp) 12150
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SCN2A Variant 1, stabilized
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    IVS intron inserted after bp4551.
  • GenBank ID
  • Entrez Gene
    SCN2A (a.k.a. BFIC3, BFIS3, BFNIS, DEE11, EA9, EIEE11, HBA, HBSCI, HBSCII, NAC2, Na(v)1.2, Nav1.2, SCN2A1, SCN2A2)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-mScarlet (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer V1-5
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SCN2A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions. Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIR-CMV-SCN2A-Variant-1-IRES-mScarlet was a gift from Al George (Addgene plasmid # 162279 ; ; RRID:Addgene_162279)
  • For your References section:

    Cryptic prokaryotic promoters explain instability of recombinant neuronal sodium channels in bacteria. DeKeyser JM, Thompson CH, George AL Jr. J Biol Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 2021 Jan 15. 10.1016/j.jbc.2021.100298 PubMed 33460646