|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162280||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6466
- Total vector size (bp) 12630
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSCN8A Variant 1, stabilized
SpeciesH. sapiens (human)
Insert Size (bp)6165
MutationModified human HSPA5 intron 4 inserted after bp2370. IVS intron inserted after bp4370. Silent codon changes at bp4208A>G and bp4211T>C
Entrez GeneSCN8A (a.k.a. BFIS5, CERIII, CIAT, DEE13, EIEE13, MED, MYOCL2, NaCh6, Nav1.6, PN4)
- Promoter CMV
/ Fusion Protein
- IRES-mScarlet (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer V1-5
- 3′ sequencing primer pcDNA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The SCN8A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions. Please visit https://www.biorxiv.org/content/10.1101/2020.11.05.370429v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet was a gift from Al George (Addgene plasmid # 162280 ; http://n2t.net/addgene:162280 ; RRID:Addgene_162280)
For your References section:Cryptic prokaryotic promoters explain instability of recombinant neuronal sodium channels in bacteria. DeKeyser JM , Thompson CH, George Jr. AL.. J Biol. Chem. (2021) 10.1016/j.jbc.2021.100298