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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
(Plasmid #162280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162280 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA4TO-IRES-mScarlet
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6466
  • Total vector size (bp) 12630
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCN8A Variant 1, stabilized
  • Alt name
    Nav1.6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6165
  • Mutation
    Modified human HSPA5 intron 4 inserted after bp2370. IVS intron inserted after bp4370. Silent codon changes at bp4208A>G and bp4211T>C
  • GenBank ID
    NM_014191
  • Entrez Gene
    SCN8A (a.k.a. BFIS5, CERIII, CIAT, EIEE13, MED, MYOCL2, NaCh6, Nav1.6, PN4)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-mScarlet (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer V1-5
  • 3′ sequencing primer pcDNA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The SCN8A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions. Please visit https://www.biorxiv.org/content/10.1101/2020.11.05.370429v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet was a gift from Al George (Addgene plasmid # 162280 ; http://n2t.net/addgene:162280 ; RRID:Addgene_162280)
  • For your References section:

    Cryptic prokaryotic promoters explain instability of recombinant neuronal sodium channels in bacteria. DeKeyser JM , Thompson CH, George Jr. AL.. J Biol. Chem. (2021) 10.1016/j.jbc.2021.100298