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pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet
(Plasmid #209411)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209411 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 209411-D50 50 μg of DNA in Tris buffer $495
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DNA 209411-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4TO-IRES-mScarlet
  • Backbone size w/o insert (bp) 6465
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCN8A Variant 3, stabilized
  • Alt name
    Nav1.6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6165
  • Mutation
    Modifed human HSPA5 intron 4 inserted after bp2370 of the SCN8A ORF. IVS intron inserted after bp4370 of the SCN8A ORF. Silent codon changes at bp4208A>G and bp4211T>C of the SCN8A ORF.
  • Entrez Gene
    SCN8A (a.k.a. BFIS5, CERIII, CIAT, DEE13, EIEE13, MED, MYOCL2, NaCh6, Nav1.6, PN4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer V1-5
  • 3′ sequencing primer pcDNA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SCN8A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet was a gift from Al George (Addgene plasmid # 209411 ; http://n2t.net/addgene:209411 ; RRID:Addgene_209411)

  • For your References section:

    Molecular and cellular context influences SCN8A variant function. Vanoye CG, Abramova TV, DeKeyser JM, Ghabra NF, Oudin MJ, Burge CB, Helbig I, Thompson CH, George AL Jr. JCI Insight. 2024 May 21;9(12):e177530. doi: 10.1172/jci.insight.177530. 10.1172/jci.insight.177530 PubMed 38771640
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