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Ian Wheeldon Lab Plasmids


The Ian Wheeldon Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica. Schwartz CM, Hussain MS, Blenner M, Wheeldon I ACS Synth Biol. 2015 Dec 29.
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica. Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I ACS Synth Biol. 2016 Dec 19.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
70007pCRISPRylCodon optimized Cas9 from S. pyogenes (Synthetic), sgRNA expression cassette (Synthetic)Yeast Expression, CRISPR, Synthetic BiologySynthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica. ACS Synth Biol. 2015 Dec 29. CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion pUC19 Add to Cart
84608pCRISPRyl_AXPCas9Yeast Expression, CRISPR, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. Introduces DSB at AXP locus in Y. lipolytica pUC19 Add to Cart
84609pCRISPRyl_XPR2Cas9Yeast Expression, CRISPR, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. Introduces DSB at XPR2 locus in Y. lipolytica pUC19 Add to Cart
84610pCRISPRyl_A08Cas9Yeast Expression, CRISPR, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. Introduces DSB at A08 locus in Y. lipolytica pUC19 Add to Cart
84611pCRISPRyl_D17Cas9Yeast Expression, CRISPR, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. Introduces DSB at D17 locus in Y. lipolytica pUC19 Add to Cart
84612pCRISPRyl_MFE1Cas9Yeast Expression, CRISPR, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. Introduces DSB at MFE1 locus in Y. lipolytica pUC19 Add to Cart
84613pHR_AXP_hrGFPhrGFPYeast Expression, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus pUC19 Add to Cart
84614pHR_XPR2_hrGFPhrGFPYeast Expression, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus pUC19 Add to Cart
84615pHR_A08_hrGFPhrGFPYeast Expression, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus pUC19 Add to Cart
84616pHR_D17_hrGFPhrGFPYeast Expression, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus pUC19 Add to Cart
84617pHR_MFE1_hrGFPhrGFPYeast Expression, Synthetic BiologyStandardized markerless gene integration for pathway engineering in Yarrowia lipolytica. ACS Synth Biol. 2016 Dec 19. HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus pUC19 Add to Cart