172,818 results

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MSCV Myc IRES GFP

Plasmid

#18770

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

Myc (Myc Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
GFP-SP140

Plasmid

#65394

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP140 (SP140 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-mTagBFP2-T2A-FLPo

Plasmid

#124834

Purpose

Monomeric TagBFP2 and FLP recombinase

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-FLPo

Expression

Mammalian

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF1917

Plasmid

#143554

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX2 (RUNX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_lenti_osTir1_9Myc_P2A_Bsd

Plasmid

#139746

Purpose

lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

Depositor

Robert Tjian

Article

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

rice E3 ligase Tir1 (osTir1)

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pNCS-mRuby3

Plasmid

#74234

Purpose

Constitutive expression of mRuby3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mRuby3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

Plasmid

#187582

Purpose

Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF7 (SRSF7 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-LY-MT-P2A

Plasmid

#214897

Purpose

Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-LY-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-U2AF2-TurboID-HA-rtTA

Plasmid

#226997

Purpose

Integrative plasmid to express U2AF2-TurboID in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Insert

U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)

Tags

TurboID-HA

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209730

Purpose

Expresses presynaptic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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sFlt1-i13 V5

Plasmid

#86044

Purpose

human Soluble Flt1 isoform i13 (terminates in intron 13)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform i13 (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform i13 (terminates in intron 13)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-GFP

Plasmid

#97151

Purpose

label apical epithelial cells in zebrafish

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

AcGFP

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH2

Plasmid

#204230

Purpose

Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH2 (MDH2 Human)

Tags

6X His

Expression

Bacterial

Mutation

Does not include transit peptide and includes a M…

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-GATA3

Plasmid

#116747

Purpose

Lentiviral expression of GATA3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GATA3 (GATA3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRXRα

Plasmid

#135910

Purpose

Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Human retinoid X receptor- alpha (RXRA Human)

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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