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pET28a-His-hAos1
(Plasmid #53135)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53135 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6496
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Aos1
  • Alt name
    SAE1
  • Alt name
    SUMO-activating enzyme subunit 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1166
  • GenBank ID
  • Entrez Gene
    SAE1 (a.k.a. AOS1, HSPC140, SUA1, UBLE1A)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-His-hAos1 was a gift from Frauke Melchior (Addgene plasmid # 53135 ; http://n2t.net/addgene:53135 ; RRID:Addgene_53135)
  • For your References section:

    The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Pichler A, Gast A, Seeler JS, Dejean A, Melchior F. Cell. 2002 Jan 11;108(1):109-20. 10.1016/S0092-8674(01)00633-X PubMed 11792325