Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #53137)


Item Catalog # Description Quantity Price (USD)
Plasmid 53137 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4107
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    protein sequence is identical to human
  • Entrez Gene
    Ube2i (a.k.a. 5830467E05Rik, UBC9, Ubce2i, Ubce9)
  • Promoter T7
  • Tag / Fusion Protein
    • untagged

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pET-RP
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-mUbc9 was a gift from Frauke Melchior (Addgene plasmid # 53137 ; ; RRID:Addgene_53137)
  • For your References section:

    The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Pichler A, Gast A, Seeler JS, Dejean A, Melchior F. Cell. 2002 Jan 11;108(1):109-20. 10.1016/S0092-8674(01)00633-X PubMed 11792325