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pET23a-mUbc9
(Plasmid #53137)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53137 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4107
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubc9
  • Alt name
    UBE2I
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    477
  • Mutation
    protein sequence is identical to human
  • Entrez Gene
    Ube2i (a.k.a. 5830467E05Rik, UBC9, Ubce2i, Ubce9)
  • Promoter T7
  • Tag / Fusion Protein
    • untagged

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pET-RP
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-mUbc9 was a gift from Frauke Melchior (Addgene plasmid # 53137 ; http://n2t.net/addgene:53137 ; RRID:Addgene_53137)
  • For your References section:

    The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Pichler A, Gast A, Seeler JS, Dejean A, Melchior F. Cell. 2002 Jan 11;108(1):109-20. 10.1016/S0092-8674(01)00633-X PubMed 11792325