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pCMV-Tag3B-CCre

Plasmid

#106369

Purpose

C-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST ERKKTRmCerulean3

Plasmid

#90229

Purpose

Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_TIA1

Plasmid

#106095

Purpose

bacterial protein expression of TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

6xHIs-thrombin and T7

Expression

Bacterial

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-SOX17

Plasmid

#104541

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGPTVII-Bar-U-MatryoshCaMP6s

Plasmid

#100024

Purpose

Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Expression

Plant

Promoter

AtUBQ10

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHL-avitag3

Plasmid

#99847

Purpose

mammalian expression with secretive signal and avi tag for biotinylation

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Avi

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MED12_pLX307

Plasmid

#98350

Purpose

Lentiviral expression of MED12

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED12 (MED12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R1392Q

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLexm

Plasmid

#99844

Purpose

mammalian expression

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMito-TrxRFP1

Plasmid

#98997

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and mitochondrial localization sequence

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pnCas9-PBE

Plasmid

#98164

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-nCas9-UGI-NLS

Expression

Plant

Promoter

Ubi

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2 (Neo)

Plasmid

#92065

Purpose

Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Insert

MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCK302

Plasmid

#87768

Purpose

As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Inserts

PrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli

Use

Synthetic Biology

Tags

6xHis Tag

Expression

Bacterial

Promoter

PrhaBAD rhamnose-inducible promoter from E. coli …

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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