|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84468||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneDerived from AAV V032
Backbone manufacturerRachael Neve
- Total vector size (bp) 9347
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)1431
MutationT230I (please see depositor comments below)
- Promoter TRE
- Cloning method Restriction Enzyme
- 5′ cloning site Asc1 (not destroyed)
- 3′ cloning site Nhe1 (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bytdTomato; from plasmid pAAV-hSyn-iCre-dTomato (JT) provided by Dr. Jonathan Ting (MIT, now at Allen Institute)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that mutation T230I in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
For more info regarding the plasmid, see RAM manuscript on eLife's homepage: http://dx.doi.org/10.7554/eLife.13918
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA was a gift from Yingxi Lin (Addgene plasmid # 84468 ; http://n2t.net/addgene:84468 ; RRID:Addgene_84468)
For your References section:A robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, Hemberg M, Yin JC, Maier SF, Lin Y. Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918. 10.7554/eLife.13918 PubMed 27661450