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172,818 results

Showing: 11461 - 11480 of 172818 results

  2. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Mutation
    None
    Promoter
    Ribo-HX1
    Available Since
    April 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62544-plasmid-map-sequence-id-109759

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  7. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  9. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  16. pLenti-Tet

    Plasmid
    #138086
    Purpose
    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles
    Depositor
    Eva Frickel
    Article
    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    minimal CMV promoter with heptamerized tet‐operat…
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138086-plasmid-map-sequence-id-276295

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  18. pSBbi-Neo

    Plasmid
    #60525
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60525-plasmid-map-sequence-id-486715

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  19. KEAP1 (KEAP1A-c029)

    Plasmid
    #110247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KEAP1 (KEAP1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110247-plasmid-map-sequence-id-212283

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Showing: 11461 - 11480 of 172818 results