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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,960 results

Showing: 11461 - 11480 of 179960 results

  1. VPS13D^EGFP

    Plasmid
    #174109
    Purpose
    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.
    Depositor
    Pietro De Camilli
    Publication
    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized VPS13D
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    With an internal EGFP tag in aminoacid position 1…
    Promoter
    CMV
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174109-plasmid-map-sequence-id-345250

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  5. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid
    #129631
    Purpose
    Visualization of Akt and ERK activity
    Depositor
    Dorus Gadella
    Publication
    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    129631-plasmid-map-sequence-id-260221

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  6. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized minidCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    171383-plasmid-map-sequence-id-337350

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  8. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    167547-plasmid-map-sequence-id-332733

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  13. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Publication
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

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  15. Sc++

    Plasmid
    #155011
    Purpose
    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sc++
    Expression
    Mammalian
    Mutation
    T1227K, Loop Substitution from S. anginosus
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    155011-plasmid-map-sequence-id-308829

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  17. LiOn*-CAG∞RFP

    Plasmid
    #154019
    Purpose
    Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Publication
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Expression
    Mammalian
    Mutation
    All TTAA in vector backbone were replaced by sile…
    Promoter
    CAG
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154019-plasmid-map-sequence-id-308117

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  20. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140973-plasmid-map-sequence-id-300655

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Showing: 11461 - 11480 of 179960 results