163,421 results

1. Lamin A (R435C)-mRFP

   Plasmid

   #124274

   Purpose

   Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R435C) (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R435C)

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Opto-zFGFR1a

   Plasmid

   #232639

   Purpose

   Zebrafish FGFR1a receptor kinase domain fused to VfLOV

   Depositor

   Katherine Rogers

   Insert

   zFGFR1a kinase domain + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-GGA1

   Plasmid

   #178459

   Purpose

   Expresses GGA1 in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

   Insert

   GGA1 (GGA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL7010

   Plasmid

   #136942

   Purpose

   Constitutive sfGFP expression (high), bacterial promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL-SUV39H2-L

   Plasmid

   #89136

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Archon1-KGC-EGFP-ER2-WPRE

   Plasmid

   #108423

   Purpose

   Archon1 fluorescent voltage reporter in mammalian expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-SLC7A11-HA

   Plasmid

   #201643

   Purpose

   Over-expression of HA-tagged human SLC7A11

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1

    Plasmid

    #192187

    Purpose

    TdgA vector

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM2314-RbcS-mNG

    Plasmid

    #165582

    Purpose

    Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

    Depositor

    Daniel Ducat

    Article

    Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Prbcs

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ypet-PBD

    Plasmid

    #105290

    Purpose

    To measure Rac or Cdc42 activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 65-150

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. G protein alpha-q-GFP

    Plasmid

    #66080

    Purpose

    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

    Depositor

    Catherine Berlot

    Article

    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

    Insert

    G protein alpha-q-EE-YFP (Gnaq Mouse)
    

    Use

    Tags

    GFP was inserted internally into the alpha-q sequ…

    Expression

    Mammalian

    Mutation

    BamHI and SacI sites in alpha-q were removed with…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrcAgBIS (CO)

    Plasmid

    #35153

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    Bisabolene Synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2333_Cas9-CtIP-dnRNF168

    Plasmid

    #200254

    Purpose

    This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

    Depositor

    Claudio Mussolino

    Article

    Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

    Insert

    Cas9-CtIP-dnRNF168
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-EF1afull-H2B-mCherry-P2A-Fluc

    Plasmid

    #207253

    Purpose

    Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    H2B-mCherry-P2A-Fluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha_full

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid

    #188524

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    2XMTS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALPS vif

    Plasmid

    #101328

    Purpose

    Encodes codon optimized NL4-3 vif

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vif
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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