pHMM
(Plasmid #67717)

• Purpose: Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein
• Depositing Labs: Robert Batey, Jeffrey Kieft
• Publication: Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.

Backbone

• Vector backbone: pET30b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5422
• Total vector size (bp): 6500
• Modifications to backbone: Insertion of sequence between NdeI and HindIII sites
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hexahistidine/Maltose binding protein/MS2 coat protein fusion
• Species: Synthetic
• Insert Size (bp): 1000
• Promoter: T7 RNA polymerase
• Tag / Fusion Protein:
  • Hexahistidine tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MS2 coat protein in this plasmid contains the V29/dIFG mutations described in Lim and Peabody 1994 (Nucleic Acids Res. 1994 Sep 11;22(18):3748-52) which are reported to prevent coat protein multimerization and increase affinity for RNA.