280,008 results
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Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
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AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVPromoterU6 and cTnTAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVPromoterU6 and cTnTAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-ccdB-Nanoluc
Plasmid#87067PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneTagsNanoluc luciferaseExpressionMammalianAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-ccdB-Nanoluc
Plasmid#87067PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneTagsNanoluc luciferaseExpressionMammalianAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
ExpressionYeastAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
ExpressionYeastAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSrc biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSrc biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R777-E139 Hs.NF1
Plasmid#70423PurposeGateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)DepositorInsertNF1 (NF1 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R777-E139 Hs.NF1
Plasmid#70423PurposeGateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)DepositorInsertNF1 (NF1 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2-GBP
ExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by