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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs

Plasmid

#106908

Purpose

full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

gag

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs

Plasmid

#106908

Purpose

full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

gag

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSSV_LTR

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSSV_LTR

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW7:1-707

Plasmid

#211099

Purpose

Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW7:1-707

Plasmid

#211099

Purpose

Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

p18

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

p18

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSDM052

Plasmid

#194341

Purpose

pUC mini-Tn7 vector expressing LseCas13a

Depositor

Joseph Bondy-Denomy

Article

Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

Insert

Cas13a(Lse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSDM052

Plasmid

#194341

Purpose

pUC mini-Tn7 vector expressing LseCas13a

Depositor

Joseph Bondy-Denomy

Article

Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

Insert

Cas13a(Lse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

Plasmid

#234454

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4f-NGR

Use

AAV; Flp/frt

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

Plasmid

#234454

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4f-NGR

Use

AAV; Flp/frt

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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