274,642 results

1. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. -5.5kb-UCP1-GFP

   Plasmid

   #104585

   Purpose

   Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

   Depositor

   Irfan Lodhi

   Article

   Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

   Insert

   UCP1 promoter (Ucp1 Mouse)
   

   Tags

   TurboGFP

   Expression

   Mammalian

   Promoter

   UCP1

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. -5.5kb-UCP1-GFP

   Plasmid

   #104585

   Purpose

   Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

   Depositor

   Irfan Lodhi

   Article

   Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

   Insert

   UCP1 promoter (Ucp1 Mouse)
   

   Tags

   TurboGFP

   Expression

   Mammalian

   Promoter

   UCP1

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ADSL_pLX307

   Plasmid

   #98311

   Purpose

   Lentiviral expression of ADSL

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   ADSL (ADSL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ADSL_pLX307

   Plasmid

   #98311

   Purpose

   Lentiviral expression of ADSL

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   ADSL (ADSL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Expression

    Mammalian

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_QLuc

    Plasmid

    #183260

    Purpose

    Express QLuc in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

    Insert

    QLuc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCSIIbleo-iRFP670-P2A-EGFP

    Plasmid

    #198060

    Purpose

    The lentiviral vector for iRFP670 and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100854

    Purpose

    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jRGECO1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100854

    Purpose

    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jRGECO1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge