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Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.
Lim B, Zimmermann M, Barry NA, Goodman AL
Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
90317pNBU2_erm_P1T_DP-GH023Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023
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90318pNBU2_erm_P1T_DP-A21Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21
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90319pNBU2_erm_P1T_DP-B1Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1
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90320pNBU2_erm_P1T_DP-rpiL*Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL*
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90321pNBU2_erm_P1T_DP-B41Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41
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90324pNBU2_erm-TetR-P1T_DP-GH023Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter
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90325pExchange_tdk_BT1311p-tetR-1Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702
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90326pExchange_tdk_BT1311p-tetR-2Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694
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90327pExchange_tdk_BT1311p-tetR-3Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957
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117727pNBU2_erm_P1T_DP-GH023 - NanoLucIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter
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117728pNBU2_erm-TetR-P1T_DP-GH023 - NanoLucIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene
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Antibodies from Article