PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90325||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Gibson Cloning
- 5′ sequencing primer GGAGAGACAGCCGAATACGA
- 3′ sequencing primer AACACTTAACGGCTGACATGG (Common Sequencing Primers)
Addgene's sequencing results found multiple mutations in ermG. The depositing lab confirmed that these mutations do not compromise erythromycin selection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pExchange_tdk_BT1311p-tetR-1 was a gift from Andrew Goodman (Addgene plasmid # 90325 ; http://n2t.net/addgene:90325 ; RRID:Addgene_90325)
For your References section:Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut. Lim B, Zimmermann M, Barry NA, Goodman AL. Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. 10.1016/j.cell.2017.03.045 PubMed 28431252