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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

Viral Prep

#107312-AAV1

Purpose

Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Tags

mCherry (physically separate, not a fusion protein)

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only
pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3M14nes

Plasmid

#155367

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Use

CRISPR

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3M14nes

Plasmid

#155367

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Use

CRISPR

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP14

Plasmid

#141267

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP14

Plasmid

#141267

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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