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167,729 results

Showing: 11561 - 11580 of 167729 results

  1. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  2. pET23a-H6-TEV-cAbl

    Plasmid
    #214234
    Purpose
    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Neel Shah
    Article
    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1
    Insert
    ABL1 Kinase domain (ABL1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214234-plasmid-map-sequence-id-422815

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  3. pCS6-YFP-SMASh(TI)

    Plasmid
    #111501
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    YFP-SMASh(TI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111501-plasmid-map-sequence-id-214268

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  7. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Article
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Expression
    Mammalian
    Promoter
    RNA polymease I Promoter
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

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  8. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  17. hSyn-DIO-somBiPOLES-mCerulean (AAV5)

    Viral Prep
    #154951-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Simon Wiegert
    Article
    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:
    Promoter
    Syn
    Tags
    mCerulean (Cre-dependent)
    Available Since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. scADGFP

    Plasmid
    #114434
    Purpose
    Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA
    Depositor
    Edward Perez-Reyes
    Article
    Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.
    Insert
    Green fluorescent protein
    Use
    AAV
    Tags
    ER export sequence FCYENEV
    Expression
    Mammalian
    Promoter
    Novel synthetic activity-dependent promoter
    Available Since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    114434-plasmid-map-sequence-id-225661

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Showing: 11561 - 11580 of 167729 results