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BirA-Myc_N_PAX6

Plasmid

#167721

Purpose

Expresses PAX6 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX6 (PAX6 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfrbeta-EGFPN

Plasmid

#66790

Purpose

Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRbeta (Pdgfrb Mouse)

Tags

C-term EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pmEGFP-DRD2

Plasmid

#190752

Purpose

Expression of DRD2 with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

DRD2 (DRD2 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

Plasmid

#188383

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a isPETase

Plasmid

#225759

Purpose

Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

isPETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) SDC3

Plasmid

#107501

Purpose

Expresses SDC3, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

SDC3 (codon optimized) (SDC3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

Plasmid

#184635

Purpose

Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v2

Plasmid

#113934

Purpose

Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

LOV2 and Stim1 polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAP

Plasmid

#166457

Purpose

Expresses fusion of mEGFP and YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pR26

Plasmid

#127372

Purpose

Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Promoter

tight TRE promoter

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Tu-His8

Plasmid

#224370

Purpose

Express EF-Tu protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Tu (tufB E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

Plasmid

#135456

Purpose

Bacterial expression of uTEV2Δ protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability, T30A/S153N mut…

Promoter

tac promoter

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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NbAlfa-GFP2

Plasmid

#201485

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbAlfa-GFP2

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBADTrfp

Plasmid

#99382

Purpose

This promoter system provided the highest RFP expression upon induction

Depositor

Nathan Hillson

Insert

RFP

Expression

Bacterial

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-mouse-Nb-Hia5

Plasmid

#239626

Purpose

Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Mouse Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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