271,642 results

1. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLG012 (DRT type 4)

   Plasmid

   #157890

   Purpose

   Expresses the DRT type 4 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 4
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLG012 (DRT type 4)

   Plasmid

   #157890

   Purpose

   Expresses the DRT type 4 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 4
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBTK527a

   Plasmid

   #110606

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBTK527a

   Plasmid

   #110606

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pEMS2159

   Plasmid

   #70120

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEMS2159

   Plasmid

   #70120

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLG013 (DRT type 5)

   Plasmid

   #157891

   Purpose

   Expresses the DRT type 5 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 5
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG013 (DRT type 5)

    Plasmid

    #157891

    Purpose

    Expresses the DRT type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 5
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

    Plasmid

    #173452

    Purpose

    Contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

    Insert

    mCherry-sfGFP-ADH1ter
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPEE47

    Plasmid

    #139426

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPEE47

    Plasmid

    #139426

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPEE46

    Plasmid

    #139425

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPEE46

    Plasmid

    #139425

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWS481

    Plasmid

    #123029

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (6x) [2a]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS481

    Plasmid

    #123029

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (6x) [2a]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLuc

    Plasmid

    #135288

    Purpose

    GBPart - GLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Gaussia Luciferase
    

    Use

    Luciferase and Synthetic Biology

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGLuc

    Plasmid

    #135288

    Purpose

    GBPart - GLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Gaussia Luciferase
    

    Use

    Luciferase and Synthetic Biology

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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