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pDONR223 SARS-CoV-2 NSP13

Plasmid

#141266

Purpose

Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP13

Plasmid

#141266

Purpose

Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTarget_CAST

Plasmid

#127926

Purpose

Target plasmid containing PSP1

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

PSP1 target (GGTT PAM)

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTarget_CAST

Plasmid

#127926

Purpose

Target plasmid containing PSP1

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

PSP1 target (GGTT PAM)

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway Cloning

Tags

mCherry and mVenus

Available since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway Cloning

Tags

mCherry and mVenus

Available since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Publication

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Publication

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Publication

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Publication

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miniTurboID

Use

Drosophila expression

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miniTurboID

Use

Drosophila expression

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Publication

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Publication

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Mutation

WT

Promoter

U6

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Mutation

WT

Promoter

U6

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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