336,748 results

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3basic luciferase and renilla_polyA

Plasmid

#128046

Purpose

pGL3 basic luciferase reporter with co-expression of renilla luciferase

Depositor

Oskar Laur

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3basic luciferase and renilla_polyA

Plasmid

#128046

Purpose

pGL3 basic luciferase reporter with co-expression of renilla luciferase

Depositor

Oskar Laur

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-EGFP (AAV2 trial size)

Viral Prep

#50465-AAV2.T

Purpose

Ready-to-use AAV2 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

Syn

Tags

EGFP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only
pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway Cloning

Tags

mCherry and mVenus

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway Cloning

Tags

mCherry and mVenus

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Article

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Article

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti HRE-Luc pGK Hygro

Plasmid

#118706

Purpose

reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HRE (HIF-responsive promoter (3xHRE)-luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti HRE-Luc pGK Hygro

Plasmid

#118706

Purpose

reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HRE (HIF-responsive promoter (3xHRE)-luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 N-term GFP tTA

Plasmid

#107551

Purpose

All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

Depositor

David Sabatini

Type

Empty backbone

Use

Lentiviral

Tags

GFP (optional)

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 N-term GFP tTA

Plasmid

#107551

Purpose

All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

Depositor

David Sabatini

Type

Empty backbone

Use

Lentiviral

Tags

GFP (optional)

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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