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pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1577
Plasmid#143133PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAHR (AHR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0490
Plasmid#143030PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZSCAN4 (ZSCAN4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgE/λ
Plasmid#50365PurposeExpresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
NeonGreen-C1-ORP5
Plasmid#220071Purposeoverexpresses ORP5 with a neonGreen fluorophoreDepositorInsertOSBPL5 (OSBPL5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag-Cas9
Plasmid#228959PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pET30a_HIV-RT
Plasmid#153311PurposeBacterial expression of HIV reverse transcriptaseDepositorInsertHIV Reverse Transcriptase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9
Plasmid#163025PurposeExpression of CRISPRi with gRNA against mouse Pcsk9DepositorInsertMammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFOK
Plasmid#166651PurposeSuicide vector for gene manipulationDepositorInsertI-sceI, sacB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1
Plasmid#65807Purposeexpression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.DepositorInsertPAB1 (PABPC1 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDY0209 ACTB Nicking guide +48
Plasmid#179109PurposeACTB N-term Nicking guide 1 +48 guideDepositorInsertACTB N-term Nicking guide 1 +48 guide
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBI
Plasmid#17816DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-enCjCas9-BlastR
Plasmid#220978PurposeExpresses human codon-optimized enCjCas9DepositorInsertenCjCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2B
Plasmid#24181PurposeMammalian expression of human JMJD2B with HA tagDepositorInsertJMJD2B (KDM4B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTags6-Histidine-tagExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-MDC1 (770-7)
Plasmid#26284DepositorInsertMediator of DNA damage checkpoint 1 (MDC1 Human)
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FLAG
Plasmid#183511PurposeGateway converted, destination vector with N-terminal Flag tag. For mammalian expression.DepositorTypeEmpty backboneUseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only
