Purpose(Empty Backbone) Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74450||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerBroad Institute
- Backbone size (bp) 10603
/ Fusion Protein
- EGFP (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
- 5′ sequencing primer hPGK-F (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Cilantro 2 was a gift from Benjamin Ebert (Addgene plasmid # 74450 ; http://n2t.net/addgene:74450 ; RRID:Addgene_74450)