165,612 results

1. p246 eSpCas9_2gRNAs_mH11

   Plasmid

   #164854

   Purpose

   gRNA vector for targeting mouse H11 locus

   Depositor

   Philip Jordan

   Article

   Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

   Insert

   gRNAs for targeting mouse H11 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ePPEplus

   Plasmid

   #205241

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

   Plasmid

   #207613

   Purpose

   vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

   Depositor

   Longzhi Tan

   Article

   Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   mouse Pcp2 promoter (L7-6)

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-NEDD8

   Plasmid

   #18711

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

   Insert

   NEDD8 (NEDD8 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5/FRT/TO (pEHA1339)

   Plasmid

   #209088

   Purpose

   Backbone plasmid for Tet Responsive transgene expression, FRT integration

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-mhYFP

   Plasmid

   #186532

   Purpose

   Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   araB

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ER-J-TAG (pGS730)

   Plasmid

   #160038

   Purpose

   pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Insert

   3xHA-sfGFP-JPH-TMD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVi U6_gRNA CMV_SadCas9-KRAB

   Plasmid

   #214609

   Purpose

   All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

   Depositor

   Ralf Gilsbach

   Article

   Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

   Inserts

   gRNA
   SadCas9
   

   Use

   AAV

   Tags

   NP NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV and U6

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWN_U6-TRAC-miniGag

   Plasmid

   #228960

   Purpose

   miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CVB3 IRES

    Plasmid

    #189968

    Purpose

    Part 2 plasmid to introduce the CVB3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    CVB3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-Flag-MIB1-IRES-Puro

    Plasmid

    #140240

    Purpose

    Lentiviral vector expressing flag-tagged MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Flag-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBtet-Bla

    Plasmid

    #60510

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-NP-B/BRISBANE/60/2008

    Plasmid

    #187921

    Purpose

    Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    UNTAGGED

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-sCAG-mBaoJin-oPRE

    Plasmid

    #218684

    Purpose

    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Short variant of the CAG promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. H2B-mGarnet2

    Plasmid

    #104312

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    H2B-mGArnet2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-V5-SBP-C1-HshnRNPQ_AA

    Plasmid

    #148251

    Purpose

    Mammalian Expression of HshnRNPQ

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPQ (SYNCRIP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge