|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67616||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonePCS2+ was modified to generate pCSf107mT
- Backbone size (bp) 4181
Modifications to backboneinserted a PCR product that facilitates GATEWAY insertions behind an in frame 3'HA tag
Vector typeMammalian Expression ; in vitro transcription or translation off of an SP6 promoter, Xenopus expression
- Promoter CMV and SP6
/ Fusion Protein
- three inframe 3' HA tags (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSF107mT-GATEWAY-3'-3HA was a gift from Todd Stukenberg (Addgene plasmid # 67616 ; http://n2t.net/addgene:67616 ; RRID:Addgene_67616)
Map uploaded by the depositor.