Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #67619)


Item Catalog # Description Quantity Price (USD)
Plasmid 67619 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    PCS2+ was modified to generate pCSf107mT
  • Backbone size (bp) 4181
  • Modifications to backbone
    inserted a PCR product that facilitates GATEWAY insertions behind an in frame 3'Flag tag
  • Vector type
    Mammalian Expression ; in vitro transcription or translation off of an SP6 promoter, Xenopus expression
  • Promoter CMV and SP6
  • Tag / Fusion Protein
    • C-terminal Flag

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSF107mT-GATEWAY-3'-FLAG was a gift from Todd Stukenberg (Addgene plasmid # 67619 ; ; RRID:Addgene_67619)
  • For your References section:

    The Xenopus ORFeome: A resource that enables functional genomics. Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist MJ. Dev Biol. 2015 Dec 15;408(2):345-57. doi: 10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29. 10.1016/j.ydbio.2015.09.004 PubMed 26391338