167,768 results

1. pMRXIP-GFP-hATG2A

   Plasmid

   #114462

   Purpose

   Expresses human ATG2A with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

   Insert

   human ATG2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   P656R (please see depositors comment below)

   Promoter

   CMV

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-APEX2-NIK3x

   Plasmid

   #129274

   Purpose

   Expression of APEX2 in the nucleolus

   Depositor

   Alice Ting

   Article

   Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

   Insert

   GFP-APEX2-NIK3x
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGloSensor -42F cGMP Vector

   Plasmid

   #236806

   Purpose

   Express -42F cGMP in biosensor assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   -42F
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC25A25

   Plasmid

   #131992

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A25 (SLC25A25 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. DUSP6

   Plasmid

   #27975

   Depositor

   Igor Astsaturov

   Article

   Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

   Insert

   DUSP6 (DUSP6 Human)
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaCTag-P19

   Plasmid

   #124789

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry-mNeonGreen
   

   Use

   Pcr template

   Tags

   mCherry-mNeonGreen

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-PGK-hESRRB

    Plasmid

    #60434

    Purpose

    Over-expression of human ESRRB

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    ESRRB (ESRRB Human)
    

    Expression

    Mammalian

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-VP6SA11

    Plasmid

    #89166

    Purpose

    Express Rotavirus SA11 VP6 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP6
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid

    #72485

    Purpose

    Express Luc2 and copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS-Antares

    Plasmid

    #74279

    Purpose

    Constitutive expression of Antares in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Antares
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1993

    Plasmid

    #239836

    Purpose

    OMEGAOff-KRK mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    CMV

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Tags

    4xCox8

    Expression

    Mammalian

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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