165,613 results

1. pMLRT

   Plasmid

   #78234

   Purpose

   Expresses Murine Leukemia Reverse Transcriptase (MLRT)

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Insert

   Murine leukemia reverse transcriptase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23102

   Available since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. IDG_TMEM63B_OE_1

   Plasmid

   #161682

   Purpose

   Overexpresses TMEM63B

   Depositor

   Mike McManus

   Insert

   TMEM63B (TMEM63B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOP-HTT-HR45Q

   Plasmid

   #92248

   Purpose

   HTT donor DNA with 45Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-CmYLCV

   Plasmid

   #205245

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human SLC KO Library

   Pooled Library

   #132552

   Purpose

   Expresses gRNAs for knockout of the human Solute Carrier family

   Depositor

   Giulio Superti-Furga

   Article

   Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

7. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDONR221_SLC25A48

   Plasmid

   #131995

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A48 (SLC25A48 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pORTMAGE-Pa1

   Plasmid

   #138475

   Purpose

   Enables high-efficiency recombineering in P. aeruginosa

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E36K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E36K

   Promoter

   Available since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2746

    Plasmid

    #144351

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HELZ2 (PRIC285 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1106

    Plasmid

    #143762

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EPAS1 (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid

    #221357

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid

    #221358

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. EC70197

    Plasmid

    #209458

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1874

    Plasmid

    #143329

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BBX (BBX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LiOn-CAG∞GFP

    Plasmid

    #154015

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasso

    Plasmid

    #207530

    Purpose

    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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