169,546 results

1. BL21(DE3) ΔserC

   Bacterial Strain

   #197656

   Purpose

   Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Bacterial Resistance

   None

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

2. pCMV5-Smad7-HA

   Plasmid

   #11733

   Depositor

   Jeff Wrana

   Article

   Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

   Insert

   SMAD7 (SMAD7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMV5-Smad7-HA

   Plasmid

   #11733

   Depositor

   Jeff Wrana

   Article

   Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

   Insert

   SMAD7 (SMAD7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pmCherry-TPX2

   Plasmid

   #31227

   Depositor

   Patricia Wadsworth

   Article

   Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

   Insert

   TPX2 (TPX2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-CAG-flex-MCS--4x6T

   Plasmid

   #196420

   Purpose

   Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

   Plasmid

   #31476

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV d14 GFP donor EF1s I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   GFP has 14 amino acids deleted from c terminus

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB.DEST-ITGB1(WT)-mRuby2

   Plasmid

   #215448

   Purpose

   Piggybac expression vector with integrin beta1 tagged with mRuby2

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Transposon-based stable expression

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   CAG

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries

   Pooled Library

   #1000000228

   Depositor

   Raj Chari

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

9. yHS1-M7A

   Plasmid

   #159151

   Purpose

   Cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   yHS1-M7A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of yHS1 to A…

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCW-Flag-CtIP-T847E

    Plasmid

    #71110

    Purpose

    mammalian expession of Flag-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR223_CFLAR_WT_V5

    Plasmid

    #82936

    Purpose

    Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CFLAR (CFLAR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid

    #72485

    Purpose

    Express Luc2 and copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pUAST-pHuji-GINKO2

    Plasmid

    #177117

    Purpose

    Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    pHuji-GINKO2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70 promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. XEC

    Plasmid

    #233341

    Purpose

    expressing SARS-CoV-2 XEC spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XEC) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T22N, F59S on the basis of KP.3

    Promoter

    CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. KP.3.1.1

    Plasmid

    #233342

    Purpose

    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (KP.3.1.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S31del on the basis of KP.3

    Promoter

    CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AID* Kit

    Plasmid Kit

    #1000000120

    Purpose

    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

    Depositor

    Helle Ulrich

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAD43-daf-16 RNAi

    Plasmid

    #34833

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only