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High-frequency, precise modification of the tomato genome.
Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
PubMed

Plasmids from Article

ID Plasmid Purpose  
70013pTC147T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.
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70014pTC150'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon
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70015pTC151TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon
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70017pTC208TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus
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70018pTC217Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
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70019pTC223Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
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