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A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79873pJMP1Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA
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79874pJMP2Bacillus subtilis sgRNA expression vector; integrates into amyE
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79875pJMP3Bacillus subtilis sgRNA expression vector; integrates into thrC
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