|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79874||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6982
- Total vector size (bp) 7572
Modifications to backboneThe rfp-targeting sgRNA RR1 was PCR-amplified from pgRNA-bacteria (Addgene #44251) using primers containing the veg promoter and EcoRI sites, digested with EcoRI, then ligated into either pDG1662 digested with EcoRI to generate pJMP2.
Vector typeBacterial Expression, CRISPR
Selectable markersBacillus subtilis chloramphenicol marker
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namesgRNA RR1
Alt namesgRNA NT
Alt nameanti-RFP sgRNA
- Promoter veg
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer agtgattatgccgcgatttc (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJMP2 was a gift from Carol Gross & Jason Peters & Stanley Qi (Addgene plasmid # 79874 ; http://n2t.net/addgene:79874 ; RRID:Addgene_79874)
For your References section:A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. 10.1016/j.cell.2016.05.003 PubMed 27238023