|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79617||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 12005
Modifications to backboneEcoRI site is mutated in Leu2
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
MutationD10A for nickase Cas9
- Promoter pGal1
/ Fusion Protein
- PmCDA1 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer caggaaacagctatgac
- 3′ sequencing primer gtaaaacgacggccagt (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThis is derived from Addgene plasmid ID43804.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS315e_pGal-nCas9(D10A)-PmCDA1 was a gift from Akihiko Kondo (Addgene plasmid # 79617 ; http://n2t.net/addgene:79617 ; RRID:Addgene_79617)
For your References section:Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. Science. 2016 Aug 4. pii: aaf8729. 10.1126/science.aaf8729 PubMed 27492474