303,613 results

1. SERPINE1-bio-His

   Plasmid

   #52077

   Purpose

   Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINE1 (SERPINE1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SERPINE1-bio-His

   Plasmid

   #52077

   Purpose

   Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINE1 (SERPINE1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #194703

   Purpose

   Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   RPS2 (RPS2 Human)
   

   Use

   Lentiviral

   Tags

   APOBEC1-HA-P2A-mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #194703

   Purpose

   Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   RPS2 (RPS2 Human)
   

   Use

   Lentiviral

   Tags

   APOBEC1-HA-P2A-mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMVV102

   Plasmid

   #215308

   Purpose

   Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMVV103

   Plasmid

   #215309

   Purpose

   Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. H6GB1tevP53SS(1-393)

   Plasmid

   #200311

   Purpose

   Bacterial expression of human superstable p53 as His6GB1tev fusion

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. H6GB1tevP53SS(1-393)

   Plasmid

   #200311

   Purpose

   Bacterial expression of human superstable p53 as His6GB1tev fusion

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-mCherry-FKBP-GEF(ARHGEF1)

    Plasmid

    #85152

    Purpose

    acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-mCherry-FKBP-GEF(ARHGEF1)

    Plasmid

    #85152

    Purpose

    acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Beta-Catenin-20

    Plasmid

    #55001

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Beta-Catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-Beta-Catenin-20

    Plasmid

    #55001

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Beta-Catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge