163,682 results

1. pLinker-6xHA-HXGPRT-LoxP

   Plasmid

   #86552

   Purpose

   The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

   Depositor

   David Sibley

   Insert

   Linker-6xHA-HGPRT-LoxP
   

   Use

   Expression in toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIPZ shTREX1

   Plasmid

   #127700

   Purpose

   Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shTREX1 (Trex1 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0831

   Plasmid

   #142208

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB33 (ZBTB33 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1180-HR-PUbECFP

   Plasmid

   #47917

   Purpose

   Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

   Depositor

   Leslie Vosshall

   Insert

   ECFP
   

   Use

   Tags

   NLS

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti polyubiquitin (PUb)

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcsII-LMP7-flag

   Plasmid

   #86773

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG2-EGFP-C-IFT144

   Plasmid

   #218740

   Purpose

   Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT144 (WDR19 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPAICS-EGFP

   Plasmid

   #99108

   Purpose

   Expresses human PAICS-EGFP fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   PAICS (PAICS Human)
   

   Use

   Tags

   EGFP (enhanced green fluorescent protein)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-SSTR3-N-17

    Plasmid

    #55135

    Purpose

    Localization: Cilium, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    SSTR3
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK-FLAG-UbxD8

    Plasmid

    #53777

    Purpose

    express FLAG-tagged human UbxD8 gene

    Depositor

    Yihong Ye

    Article

    Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

    Insert

    UbxD8 (FAF2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PL-SIN-Oct4-EGFP

    Plasmid

    #21319

    Purpose

    Lentiviral vector, expresses EGFP under Oct3/4 promoter

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Oct-4 promoter region

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid

    #203350

    Purpose

    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Molly Stevens, Christopher Grigsby

    Insert

    Nano Luciferase
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIBN-EYFP-hLaminB1

    Plasmid

    #103800

    Purpose

    BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. XEC

    Plasmid

    #233341

    Purpose

    expressing SARS-CoV-2 XEC spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XEC) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T22N, F59S on the basis of KP.3

    Promoter

    CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. KP.3.1.1

    Plasmid

    #233342

    Purpose

    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (KP.3.1.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S31del on the basis of KP.3

    Promoter

    CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-SADB19G

    Plasmid

    #32633

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. sAB-K29

    Plasmid

    #204735

    Purpose

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

    Depositor

    Minglei Zhao

    Article

    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

    Insert

    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge