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Lenti-multi-CRISPR

Plasmid

#85402

Purpose

Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTNS1

Plasmid

#64967

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Available Since

Oct. 23, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pON.mCherry

Plasmid

#84821

Purpose

Constitutive Expression plasmid with mCherry fluorescent protein

Depositor

Howard Shuman

Article

Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

Insert

mCherry

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRpHLuorin2-N1

Plasmid

#171716

Purpose

Ratiometric pHLuorin2 backbone (C terminal fusion)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Type

Empty backbone

Expression

Mammalian

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ132C

Plasmid

#69285

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of TagBFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

Plasmid

#216323

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

Plasmid

#216322

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MEF2D_SS18

Plasmid

#205853

Purpose

Express mEGFP-tagged fusion protein, MEF2D_SS18 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MEF2D_SS18 (MEF2D, SS18 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MEF2D_HNRNPUL1

Plasmid

#205852

Purpose

Express mEGFP-tagged fusion protein, MEF2D_HNRNPUL1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MEF2D_HNRNPUL1 (MEF2D, HNRNPUL1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MEF2D_DAZAP1

Plasmid

#205851

Purpose

Express mEGFP-tagged fusion protein, MEF2D_DAZAP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MEF2D_DAZAP1 (MEF2D, DAZAP1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/mit-2mutAEQ

Plasmid

#45539

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

mit-2mutAEQ

Tags

Mitochondrially targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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EC71174

Plasmid

#154067

Purpose

Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Inserts

HS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette

Use

Cre/Lox and Synthetic Biology; Golden gate

Expression

Bacterial and Plant

Mutation

The Arabidopsis thaliana U5 small nuclear ribonuc…

Promoter

35S promoter, Hordeum vulgare HSP 17 promoter use…

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF0499

Plasmid

#143881

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PPARGC1A (PPARGC1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTrex-n-eGFP

Plasmid

#62544

Purpose

Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

eGFP

Use

(1)trypanosoma cruzi expression of egfp (2) genom…

Tags

None

Mutation

None

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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