169,546 results

1. p426_Cas9_gRNA-CAN1y

   Plasmid

   #87391

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. HLA_A2402

   Plasmid

   #165010

   Purpose

   Expression of HLA-A*24:02 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-dCas9-HA-NLS--GFP-p300

   Plasmid

   #183925

   Purpose

   p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-p300
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Bsd-CMV-MDM4

   Plasmid

   #202669

   Purpose

   MDM4 overexpression vector with blasticidin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 regulator of p53 (MDM4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/SB10

   Plasmid

   #24551

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeptune2-C1

   Plasmid

   #54836

   Purpose

   Localization: C1 Cloning Vector, Excitation: 600, Emission: 650

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mNeptune2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Strep-His-Tev-hCGN

   Plasmid

   #236512

   Purpose

   For expression of Cingulin (human) in insect cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGN (CGN Human)
   

   Use

   Tags

   2xStrep-10xHis-Tev

   Expression

   Insect

   Mutation

   WT

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

   Plasmid

   #230509

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIP1

    Plasmid

    #233685

    Purpose

    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

    Depositor

    Christopher Bahl

    Insert

    Ulp1-like SUMO protease
    

    Use

    Tags

    CAHS-107838 and HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. SJZ5-HPH

    Plasmid

    #228258

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    hph
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1 S.c.

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR/zeo-MACROD1

    Plasmid

    #172572

    Purpose

    For gateway cloning of N-terminal tagged MACROD1 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_RTN3E-mScarlet3

    Plasmid

    #216255

    Purpose

    mammalian expression vector for RTN3E-mScarlet3

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon 3 isoform e (RTN3 Human)
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPOTv7-blast-mStayGold[E138D]

    Plasmid

    #221049

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mStayGold[E138D]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Human, Mouse)
    TRAC-3' homology arm (TRAC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA304

    Plasmid

    #216072

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. scQers promoter control #1 (low: minimal promoter, p027)

    Pooled Library

    #217420

    Purpose

    scQers promoter control #1 (low: minimal promoter, p027)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

19. pUPD2-HR1bR181A R185A-C (B3-B4)

    Plasmid

    #211855

    Purpose

    CDS for mutant HR1b degron in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    R181A R185A

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211861

    Purpose

    Nuclear-localized E3-DART ligase in domestication vector.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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