167,945 results

1. pAIP4_2ESK

   Plasmid

   #234232

   Purpose

   Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2ESK (UBE2D2 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. DEXsynth:eGFP-ccdb + mcherry

   Plasmid

   #233311

   Purpose

   GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   DEXsynth:eGFP-ccdb + mcherry
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tom20-ECFP-FRB

   Plasmid

   #210289

   Purpose

   mitochondrial OM marker and "anchor" in FKBP-FRB CID

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   Tom20-ECFP-FRB
   

   Tags

   ECFP and FRB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL1029

   Plasmid

   #225600

   Purpose

   The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

   Depositor

   Yong Zhang

   Article

   Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-pDEST_BirA_Flag

   Plasmid

   #227960

   Purpose

   BioID experiment

   Depositor

   Alessandro Ori

   Insert

   BirA
   

   Expression

   Mammalian

   Mutation

   R118G

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Tags

   V5-His

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti puro HA-H2BC21

   Plasmid

   #227589

   Purpose

   Lentiviral plasmid expressing human H2BC21 protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   H2BC21 (H2BC21 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CMV:Flag-CeNL

   Plasmid

   #208839

   Purpose

   Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   CeNL Nanolantern
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA012

   Plasmid

   #215974

   Purpose

   Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GRM6-DuET

    Plasmid

    #213304

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM6 (GRM6 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL0_29 [aph(3')-I EZ-Tn5]

    Plasmid

    #198942

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Charlie Gilbert, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    aph(3')-I EZ-Tn5
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCBP60g-NLS-3xmCitrine

    Plasmid

    #192521

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    CALMODULIN BINDING PROTEIN 60g
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    CBP60g

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LibTest

    Pooled Library

    #113147

    Purpose

    Customized sgRNA library targeting 86 E. coli genes

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Expression

    Bacterial

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pCMV_53BP1_FLAG

    Plasmid

    #237345

    Purpose

    transient overexpression in mammalian cells

    Depositor

    Michele Pagano

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC005.em

    Plasmid

    #182738

    Purpose

    CRISPR-Cas12a genome editing of E. faecium

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-13Myc-kanMX6

    Plasmid

    #39294

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK445 (BSD-P2A-BromoTag)

    Plasmid

    #214375

    Purpose

    BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn-DIO-somBiPOLES-mCerulean (AAV5)

    Viral Prep

    #154951-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Promoter

    Syn

    Tags

    mCerulean (Cre-dependent)

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221_SLC34A2

    Plasmid

    #132088

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC34A2 (SLC34A2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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