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176,748 results

Showing: 11861 - 11880 of 176748 results

  6. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

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  7. pGBW-m4137384

    Plasmid
    #149543
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    H. sapiens recode
    Promoter
    CMV
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149543-plasmid-map-sequence-id-306267

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  8. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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  13. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133885-plasmid-map-sequence-id-274419

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  15. CIDAR MoClo Extension, Volume I

    Plasmid Kit
    #1000000161
    Purpose
    Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV Retrograde)

    Viral Prep
    #104495-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    CAG
    Available Since
    Oct. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. HA-Rab7A

    Plasmid
    #131417
    Purpose
    Expresses HA-tagged human Rab7A in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Rab7A
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131417-plasmid-map-sequence-id-258194

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Showing: 11861 - 11880 of 176748 results