167,945 results

1. p4xSTAT6-Luc2P

   Plasmid

   #35554

   Depositor

   Axel Nohturfft

   Insert

   4xStat6 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   4xStat6

   Available Since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-Halo,IRES-eGFP

   Plasmid

   #164519

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab7A

   Plasmid

   #28047

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. STK11 gRNA (BRDN0001146880)

   Plasmid

   #75912

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK11

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK11 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Foff 2.0-EYFP (AAV8)

   Viral Prep

   #137162-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-EYFP (#137162). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-EYFP plasmid DNA. EF1a-driven expression of EYFP in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-NES-PhoCl2f-mCherry

   Plasmid

   #164048

   Purpose

   Optogenetic control of protein localization by PhoCl2f

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2f-mCherry
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDC32-HPB

    Plasmid

    #32078

    Depositor

    Fumi Katagiri

    Article

    Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

    Type

    Empty backbone

    Use

    Gateway destination binary vector

    Tags

    HA-Prescission-Biotin

    Promoter

    35S promoter

    Available Since

    Dec. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-beta-HA-BRCA2

    Plasmid

    #133553

    Purpose

    Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

    Depositor

    David Livingston

    Article

    Chen et al Mol Cell. 1998 Sep;2(3):317-28.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.DEST.EGFP

    Plasmid

    #32684

    Purpose

    Lentiviral cDNA/shRNA gateway destination vectors

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-EGFP-FLAG-PSMD2

    Plasmid

    #161917

    Purpose

    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX302 GDF11-V5 puro

    Plasmid

    #83097

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. AAT-PB-CD2APtk

    Plasmid

    #86004

    Purpose

    homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Insert

    DsRedEx_T2A_puro_dTK
    

    Use

    Piggybac exciseable

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Tol2

    Plasmid

    #31823

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-ERK2

    Plasmid

    #118296

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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