163,765 results
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Plasmid#141355PurposepMP71 expression vector containing BFPDepositorInserttagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertPPP2CB (PPP2CB Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hcGAS(157-522)
Plasmid#108676PurposeFor expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522DepositorInsertcGAS amino acid 157-522 (CGAS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acid 157-522PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PRKAR1B
Plasmid#23376DepositorInsertPRKAR1B (PRKAR1B Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
AttB_mCherry
Plasmid#171598PurposeStrong mCherry fluorescence from Bxb1 landing padDepositorInsertmCherry
UseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-OCT4
Plasmid#130692PurposeLentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.DepositorInserthuman OCT4 (POU5F1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AARS_C1_pNIC-Bio3
Plasmid#153046PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorInsertAARS (AARS Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SmoM2 (W535L)-pcw107
Plasmid#64627Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertSMO (SMO Human)
UseLentiviralTagsExpressionMutationW535LPromoterPGKAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPSdown
Plasmid#149417PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-H2B-mTagBFP2
Plasmid#161791Purpose3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; MulticistronicTagsEGFP and mTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbCAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_AAV
Plasmid#119924PurposeAll-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassetteDepositorInsertNme2Cas9
UseAAVTagsNLS-3xHA-NLS and NLS-NLSExpressionMammalianMutationPromoterU1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
Plasmid#31476DepositorInsertSFFV d14 GFP donor EF1s I-SceI
UseLentiviralTagsHA and NLSExpressionMutationGFP has 14 amino acids deleted from c terminusPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPSup2
Plasmid#149418PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only