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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LeuRS-His

Plasmid

#124113

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Leucyl-tRNA ligase (leuS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LeuRS-His

Plasmid

#124113

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Leucyl-tRNA ligase (leuS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSAR-MT-APC

Plasmid

#16487

Depositor

Bert Vogelstein

Article

Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

Insert

APC (APC Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pSAR-MT-APC

Plasmid

#16487

Depositor

Bert Vogelstein

Article

Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

Insert

APC (APC Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Article

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD

Plasmid

#171238

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD

Plasmid

#171238

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ14101 aeBlue

Plasmid

#239453

Purpose

express aeBlue chromoprotein

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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