165,637 results

1. QUAS-5-T7-GFP_ColE1

   Plasmid

   #171653

   Purpose

   QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-5-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. QUAS-10-T7-GFP_ColE1

   Plasmid

   #171654

   Purpose

   QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-10-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. QUAS-15-T7-GFP_ColE1

   Plasmid

   #171655

   Purpose

   QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-15-T7
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIH-tag RFPt- Rab11a WT

   Plasmid

   #161992

   Purpose

   Retroviral expression vector containing Rab11a tagged with RFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   RAB11A (RAB11A Canis Lupus)
   

   Use

   Retroviral

   Tags

   RFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_mNeongreen

   Plasmid

   #182413

   Purpose

   Bacterial expression of mNeongreen

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

   Plasmid

   #74928

   Purpose

   Translation reporter

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-kif18b-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED-mScarlet3

   Plasmid

   #219640

   Purpose

   Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-nSpCas9-evoCDA1

   Plasmid

   #209042

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   evoCDA1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVITRO1-dV-IgE/λ

   Plasmid

   #61879

   Purpose

   Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin lambda constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. hNET pcDNA3

    Plasmid

    #15475

    Depositor

    Randy Blakely

    Article

    Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

    Insert

    hNET (SLC6A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid

    #141385

    Purpose

    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-E (E SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-TurboID-EGFP

    Plasmid

    #163846

    Purpose

    In vitro transcription of TurboID-EGFP. Parton lab clone JRS

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTfR-mScarlet-I

    Plasmid

    #112954

    Purpose

    In vivo visualization of Transferrin receptors (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Insert

    pTfR
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKO403_lacZ_Cm

    Plasmid

    #174725

    Purpose

    Bifidobacterium-E.coli shuttle vector for seamless gene cloning

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163059

    Purpose

    mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge