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pRH2521
(Plasmid #84380)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84380 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTE-10M-0X
  • Backbone manufacturer
    Addgene 20318
  • Backbone size w/o insert (bp) 6608
  • Total vector size (bp) 6638
  • Modifications to backbone
    The sequence Pmyc1tetO (2) followed by 2 BbsI sites and the sgRNA sequences were synthesized and cloned in pTE-10M-0X at a ClaI site to produce pRH2521
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sgRNA cloning site
  • Insert Size (bp)
    30

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRH2521 was a gift from Robert Husson (Addgene plasmid # 84380)
  • For your References section:

    Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system. Singh AK, Carette X, Potluri LP, Sharp JD, Xu R, Prisic S, Husson RN. Nucleic Acids Res. 2016 Jul 12. pii: gkw625. 10.1093/nar/gkw625 PubMed 27407107