PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104622||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Promoter hsp60
- Cloning method Gibson Cloning
Shuttle vector Mycobacterium smegmatis/E.coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSBY1_FnCpf1cg was a gift from Sheng Yang (Addgene plasmid # 104622 ; http://n2t.net/addgene:104622 ; RRID:Addgene_104622)
For your References section:A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y. Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6. 10.1002/biot.201700588 PubMed 30039929