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A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis.
Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y
Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104622pSBY1_FnCpf1cgCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1
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104623pSBY2_ligD(FnCpf1)CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD
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104624pSBY3_ligDCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
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104625pSBY3_ligD-HygRCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
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